ChIP analysis was performed following the procedure described previously [36 (link)–38 (link)]. Briefly, cells in 10-cm plates were washed with PBS, cross-linked with 1% formaldehyde, lysed, and sonicated (XL-2000; QSonica LLC). IP was then performed in the cleared lysate with 5 μg of FLAG antibody (Sigma, for FLAG-α-Syn) or control IgG (Santa Cruz Biotechnology, Inc.) and Magna ChIP Protein A magnetic beads (Millipore, catalog no. 16–661) overnight. After washing the IP beads, protein-DNA complexes were eluted and the cross-links were reversed. DNA was purified using standard phenol/chloroform extraction and finally dissolved in 10 mM Tris-HCl (pH 8). The ChIP and 1% of the input DNA were subjected to SYBR Green-based real-time PCR (7500 Real-Time PCR System; Applied Biosystems) with appropriate primers (Supplementary Table S1) and SYBR Premix Ex Taq (TaKaRa). ChIP data were calculated as percent input.