Diagnostic Assays for Dengue Virus

Patients' clinical blood parameters were tested using the standard protocols in the Department of Pathology, NCKUH. Total circulating white blood cell counts (WBCs), platelet counts, and hematocrit were obtained by the standard complete blood count test. In addition, a DENV diagnosis was confirmed using one or more examinations: positive for plasma nonstructural protein 1 (NS1) antigen, dengue IgM antibodies detected using a kit (Bioline Dengue Duo™; Standard Diagnostics, Seoul, Korea), or DENV RNA detected using real-time reverse transcriptase-polymerase chain reaction (RT-PCR) (TIB Molbiol, Lightmix kit; Roche Applied Science, Berlin, Germany) [26 (link)]. For viral NS1 measurement, NS1 ELISA was performed using paired anti-NS1 antibodies that were prepared in our laboratory, and the ELISA was quantified by the addition of 100 μL of 3,3′,5,5′-tetramethylbenzidine (TMB) substrate (R&D Systems) [27 (link)].

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Nanda J.D., Jung C.J., Satria R.D., Jhan M.K., Shen T.J., Tseng P.C., Wang Y.T., Ho T.S, & Lin C.F. (2021). Serum IL-18 Is a Potential Biomarker for Predicting Severe Dengue Disease Progression. Journal of Immunology Research, 2021, 7652569.

Publication 2021

3,3′,5,5′-tetramethylbenzidine (TMB) substrate

Anti antibodies Antigen Blood Complete blood count Dengue Diagnostics Elisa Examinations Hematocrit Igm antibodies Patients Plasma protein Platelet counts Protein plasma Real time polymerase chain reaction Reverse transcriptase Rt pcr Tetramethylbenzidine White blood cell counts

Corresponding Organization : Chest Hospital, Ministry of Health and Welfare

Other organizations : Universitas Gadjah Mada, Rumah Sakit Umum Pusat Dr. Sardjito

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Variable analysis

independent variables

DENV diagnosis confirmed using one or more examinations: positive for plasma nonstructural protein 1 (NS1) antigen, dengue IgM antibodies detected, or DENV RNA detected using real-time reverse transcriptase-polymerase chain reaction (RT-PCR)

dependent variables

Total circulating white blood cell counts (WBCs)
Platelet counts
Hematocrit
Viral NS1 measurement using NS1 ELISA

control variables

Patients' clinical blood parameters were tested using the standard protocols in the Department of Pathology, NCKUH

positive controls

Paired anti-NS1 antibodies that were prepared in the laboratory for the NS1 ELISA

negative controls

No explicit negative controls mentioned

Annotations

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