Nicotine bitartrate (Sigma-Aldrich) was dissolved in 0.9% sterile saline (pH 7.4) and passed through a microfilter. A low dose (5 μg/kg/infusions) of nicotine was selected based on previous work demonstrating that this dose engenders rapid rates of acquisition, high levels of self-administration under extended access conditions, and robust levels of subsequent nicotine-seeking following 10 days of abstinence (Sanchez et al., 2013 (link); 2014 (link)). The dose is expressed as the free base weight and infusions were delivered at a rate of 0.1 ml/sec based on the animal’s weight. Nicotine solution was stored at 4 °C but was available at room temperature during self-administration sessions.
Protocol detail
Nicotine Self-Administration Protocol
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Corresponding Organization : University of Virginia
Other organizations : Virginia Commonwealth University
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Variable analysis
independent variables
- Nicotine dose (5 μg/kg/infusions)
- Rates of acquisition of nicotine self-administration
- Levels of nicotine self-administration under extended access conditions
- Levels of subsequent nicotine-seeking following 10 days of abstinence
- PH of the saline solution (7.4)
- Infusion rate (0.1 ml/sec)
- Temperature of the nicotine solution (stored at 4 °C, available at room temperature during self-administration sessions)
- Positive control: Not specified
- Negative control: Not specified
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