Zika Virus Detection in Florida

Clinical samples from locally-acquired ZIKV infections were collected from June 22 to October 11, 2016. The Florida DOH identified persons with compatible illness and clinical samples were shipped to the Bureau of Public Health Laboratories for confirmation by qRT-PCR and antibody tests following interim guidelines^{3 (link),38 (link)–40 (link)}. Clinical specimens (whole blood, serum, saliva, or urine) submitted for analysis were refrigerated or frozen at ≤ −70°C until RNA was extracted. RNA was extracted using the RNAeasy kit (QIAGEN), MagMAX for Microarrays Total RNA Isolation Kit (Ambion), or MagNA Pure LC 2.0 or 96 Systems (Roche Diagnostics). Purified RNA was eluted into 50–100 μL using the supplied elution buffers, immediately frozen at ≤ −70°C, and transported on dry ice. The Florida DOH also provided investigation data for these samples, including symptom onset dates and, when available, assignments to the zone where infection likely occurred (Supplementary Table 1).

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Grubaugh N.D., Ladner J.T., Kraemer M.U., Dudas G., Tan A.L., Gangavarapu K., Wiley M.R., White S., Thézé J., Magnani D.M., Prieto K., Reyes D., Bingham A., Paul L.M., Robles-Sikisaka R., Oliveira G., Pronty D., Barcellona C.M., Metsky H.C., Baniecki M.L., Barnes K.G., Chak B., Freije C.A., Gladden-Young A., Gnirke A., Luo C., MacInnis B., Matranga C.B., Park D.J., Qu J., Schaffner S.F., Tomkins-Tinch C., West K.L., Winnicki S.M., Wohl S., Yozwiak N.L., Quick J., Fauver J.R., Khan K., Brent S.E., Reiner RC J.r., Lichtenberger P.N., Ricciardi M., Bailey V.K., Watkins D.I., Cone M.R., Kopp EW I.V., Hogan K.N., Cannons A.C., Jean R., Monaghan A.J., Garry R.F., Loman N.J., Faria N.R., Porcelli M.C., Vasquez C., Nagle E.R., Cummings D.A., Stanek D., Rambaut A., Sanchez-Lockhart M., Sabeti P.C., Gillis L.D., Michael S.F., Bedford T., Pybus O.G., Isern S., Palacios G, & Andersen K.G. (2017). Genomic epidemiology reveals multiple introductions of Zika virus into the United States. Nature, 546(7658), 401-405.

Publication 2017

RNAeasy kit MagMAX for Microarrays Total RNA Isolation Kit MagNA Pure LC 2.0 or 96 Systems

Antibody Blood Buffers Diagnostics Dry ice Frozen Infection Isolation Microarrays Saliva Serum Urine Zikv infections

Corresponding Organization : Scripps Research Institute

Other organizations : United States Army Medical Research Institute of Infectious Diseases, University of Oxford, Fred Hutch Cancer Center, Florida Gulf Coast University, Florida Department of Health, University of Miami, Broad Institute, University of Birmingham, Colorado State University, St. Michael's Hospital, University of Washington, Institute for Health Metrics and Evaluation, Miami Dade College, NSF National Center for Atmospheric Research, Tulane University, Miami-Dade Public Library System, University of Nebraska Medical Center, University of Florida, University of Edinburgh, Harvard University, Center for Systems Biology

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Confirmation of ZIKV infection by qRT-PCR and antibody tests

control variables

None explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

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