Protocol detail

Autologous Porcine Mesenchymal Stem Cell Isolation and Extracellular Vesicle Characterization

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Eirin A., Riester S.M., Zhu X.Y., Tang H., Evans J.M., O'Brien D., van Wijnen A.J, & Lerman L.O. (2014). MicroRNA and mRNA Cargo of Extracellular Vesicles from Porcine Adipose Tissue-Derived Mesenchymal Stem Cells. Gene, 551(1), 55-64.

Publication 2014

advanced MEM medium Gibco Cell Culture Freezing Medium

Abdominal fat Adipose tissue Animal Cd44 Cd73 Cd90 Cell Cell culture procedures Centrifugation Collagenase Domestic pigs Exosome Female Hepes Human Immuno fluorescent Integrins Microvesicle Phenotype Platelet Porcine Serum Supplements Tissue Ultracentrifugation

Corresponding Organization :

Other organizations : Mayo Clinic, Mayo Clinic in Arizona, Mayo Clinic in Florida

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