Western Blot Analysis of CAPS1 and PCNA

Protein expression of CAPS1 and PCNA was detected by Western blotting, as previously described [24 (link)]. Briefly, cells were lysed using radioimmunoprecipitation assay (RIPA) buffer and subjected to immunoblotting using anti-CAPS1 (PRS4561; Sigma-Aldrich, St. Louis, MO, USA), anti-PCNA (AB92552; Abcam, Cambridge, UK), and anti-GAPDH (AB181603; Abcam) antibodies. Proteins were visualized using an enhanced chemiluminescence kit (Pierce, Brookline, MA, USA).

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Xue R., Tang W., Dong P., Weng S., Ma L., Chen S., Liu T., Shen X., Huang X., Zhang S, & Dong L. (2016). CAPS1 Negatively Regulates Hepatocellular Carcinoma Development through Alteration of Exocytosis-Associated Tumor Microenvironment. International Journal of Molecular Sciences, 17(10), 1626.

Publication 2016

AB92552 AB181603

Antibodies Buffer Caps1 Cells Chemiluminescence Gapdh Pcna Proteins Radioimmunoprecipitation assay

Corresponding Organization : Zhongshan Hospital

Other organizations : Shanghai Medical College of Fudan University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein expression of CAPS1
Protein expression of PCNA

control variables

GAPDH expression

controls

Positive control: None mentioned
Negative control: None mentioned

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