The small interfering RNAs (siRNAs) used in this study were: All Star Negative Control siRNA (QIAGEN, Hilden, Germany; Cat# 1027280), human siSTAU1: 5′-CCUAUAACUACAACAUGAGdTdT-3′,11 (link),15 (link)–17 (link) and siMTOR: 5′-GAG CCUUGUUGAUCCUUAA-3′.19 (link) All siRNA oligonucleotides were synthesized by Invitrogen (Carlsbad, CA). The oligonucleotides were deprotected, and the complementary strands were annealed. Bafilomycin A1 (Baf; InvivoGen, San Diego, CA; Cat# tlrl-baf1), rapamycin (InvivoGen, Cat# tlrl-rap), thapsigargin (Tocris, Ellisville, MO; Cat# 1138), tunicamycin (Tocris, Cat# 3516), Ionomycin (Tocris, Cat# 1704), and sodium arsenite solution (Sigma-Aldrich, St Louis, MO; Cat# 1062771000) were used in this study.
Protocol detail
siRNA Knockdown of STAU1 and mTOR
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Bafilomycin a1
Human
Ionomycin
Oligonucleotides
Rapamycin
Sirna
Sodium arsenite
Thapsigargin
Tunicamycin
Corresponding Organization : University of Florida
Top 5 similar protocols
Variable analysis
independent variables
- All Star Negative Control siRNA
- SiSTAU1
- SiMTOR
- Bafilomycin A1 (Baf)
- Rapamycin
- Thapsigargin
- Tunicamycin
- Ionomycin
- Sodium arsenite solution
- Not explicitly mentioned
- Not explicitly mentioned
- Positive control: All Star Negative Control siRNA
- Negative control: Not specified
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!