Bacterial community composition in isolated DNA samples was characterized by amplification of the V1-3 (forward, 8f:5′-AGAGTTTGATCMTGGCTCAG-3′; reverse, 518r:5′-ATTACCGCGGCTGCTGG-3′) variable region of the 16S rRNA gene by polymerase chain reaction (PCR), as previously described (43 (link)). 16S rRNA PCR products were quantified, pooled, and purified for the sequencing reaction. Sequencing was performed on a 454 Life Sciences Genome Sequencer FLX machine (Roche, Florence, SC, USA) by the Microbiome Core Facility in the UNC School of Medicine.
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Profiling Bacterial Community using 16S rRNA
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Corresponding Organization : Karolinska Institutet
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Variable analysis
independent variables
- Bacterial community composition in isolated DNA samples
- 16S rRNA gene amplification
- 16S rRNA PCR product quantification and pooling
- 16S rRNA sequencing
- V1-3 variable region of the 16S rRNA gene used for amplification
- Previously described protocol for 16S rRNA gene amplification (43)
- No positive or negative controls are explicitly mentioned in the input.
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