The liver and aorta from a representative rat in each group were fixed in 10% buffered formalin, embedded in paraffin, and cut into 5-μm sections. Certain sections were stained with hematoxylin and eosin (H&E) for histological examination of fat droplets. Samples of aorta were stained with Oil red O, as previously described (16 (link)). Images were acquired using an SZX10 microscope (Olympus; Tokyo, Japan).
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Histological Analysis of Rat Liver and Aorta
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Corresponding Organization :
Other organizations : Sangji University, Kyung Hee University, Kolmar Korea (South Korea)
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Variable analysis
independent variables
- None explicitly mentioned
- Histological examination of fat droplets in liver sections
- Oil red O staining of aorta samples
- Rat as the experimental animal model
- Tissue samples (liver and aorta) from a representative rat in each group
- Fixation of tissues in 10% buffered formalin
- Embedding in paraffin
- Cutting of sections into 5-μm thickness
- Staining of sections with hematoxylin and eosin (H&E) and Oil red O
- Positive control: Not specified
- Negative control: Not specified
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