Recombinant human growth hormone (Genotropin, somatropin 1.3 mg/4iU inj.) was obtained from Pharmacia & Upjohn Co, Div of Pfizer Inc. (NY). Bovine serum albumin was obtained from Sigma Chemical Co. (St Louis, Missouri). Dipotassium hydrogen phosphate (K2HPO4) and potassium dihydrogen phosphate (KH2PO4) were purchased from El-Nasr Chemical Co (Egypt). Glutathione peroxidase and reductase assay kit were purchased from Randox Laboratories (Antrim, UK) and Trevigen, Inc. (Gaithersburg, Maryland), respectively. Mag-NA Pure Compact RNA Isolation Kit and LightCycler_RNA Master SYBR Green I were obtained from Roche Diagnostics, (Indianapolis, Indiana)/Roche Applied Science (Mannheim, Germany). Beta-actin primer sequence design was carried out according to Peinnequin and coworkers [26 (link)], while other primer sequences were designed according to Loh and his colleagues [27 (link)]. All other chemicals and solvents were of highest-grade commercial available.
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