Recombinant Human Growth Hormone Protocol

Recombinant human growth hormone (Genotropin, somatropin 1.3 mg/4iU inj.) was obtained from Pharmacia & Upjohn Co, Div of Pfizer Inc. (NY). Bovine serum albumin was obtained from Sigma Chemical Co. (St Louis, Missouri). Dipotassium hydrogen phosphate (K2HPO4) and potassium dihydrogen phosphate (KH2PO4) were purchased from El-Nasr Chemical Co (Egypt). Glutathione peroxidase and reductase assay kit were purchased from Randox Laboratories (Antrim, UK) and Trevigen, Inc. (Gaithersburg, Maryland), respectively. Mag-NA Pure Compact RNA Isolation Kit and LightCycler_RNA Master SYBR Green I were obtained from Roche Diagnostics, (Indianapolis, Indiana)/Roche Applied Science (Mannheim, Germany). Beta-actin primer sequence design was carried out according to Peinnequin and coworkers [26 (link)], while other primer sequences were designed according to Loh and his colleagues [27 (link)]. All other chemicals and solvents were of highest-grade commercial available.

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Mahran Y.F., El-Demerdash E., Nada A.S., El-Naga R.N., Ali A.A, & Abdel-Naim A.B. (2015). Growth Hormone Ameliorates the Radiotherapy-Induced Ovarian Follicular Loss in Rats: Impact on Oxidative Stress, Apoptosis and IGF-1/IGF-1R Axis. PLoS ONE, 10(10), e0140055.

Publication 2015

Bovine serum albumin Glutathione peroxidase Mag-NA Pure Compact RNA Isolation Kit

Assay Beta actin Bovine serum albumin Diagnostics Genotropin Glutathione peroxidase Isolation K2hpo4 Potassium dihydrogen phosphate Primer Randox Recombinant human growth hormone Reductase Solvents Somatropin Sybr green i

Corresponding Organization : Al-Azhar University

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Variable analysis

independent variables

Recombinant human growth hormone (Genotropin, somatropin 1.3 mg/4iU inj.)

dependent variables

Not explicitly mentioned

control variables

Bovine serum albumin
Dipotassium hydrogen phosphate (K2HPO4)
Potassium dihydrogen phosphate (KH2PO4)
Glutathione peroxidase and reductase assay kit
Mag-NA Pure Compact RNA Isolation Kit
LightCycler_RNA Master SYBR Green I
Beta-actin primer sequence design
Other primer sequences

positive controls

Not specified

negative controls

Not specified

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