When cells were treated with WP1130 for 12 h or cells were knocked down of c-Maf or USP5 for 48–72 h, cells were collected for Annexin V-FITC/PI staining as the instructions from the manufacturer (MultiSciences Biotech Co., Ltd, Hangzhou, China) and subjected to analysis on a BD flow cytometer as described previously.10 (link)
Free full text: Click here