Human PCa LNCaP, 22Rv1, PC3, and PWR-1E cells were purchased from ATCC (Manassas, VA). MDA PCa 2b cells were from Dr. Balaji (Wake Forest School of Medicine). RC77T/E and RC77N/E cell lines characterization was reported by Theodore et al.64 (link) E006AA-hT cells were from Dr. Koochekpour (Roswell Park Memorial Institute). ExoQuick™ Kit was from System Biosciences (Mountain View, CA). CD63 (#ab59479), actin (#ab8226), and Rab5 (#ab18211) antibodies were from Abcam (Cambridge, MA). Secondary antibodies anti-rabbit IgG (H + L) F(ab’)2 fragment (Alexa Fluor® 555 Conjugate) (#4413 S), anti-mouse IgG (H + L) F(ab’)2 fragment (Alexa Fluor® 647 Conjugate) (#4410 S), and anti-mouse IgG (H + L) F(ab’)2 fragment (Alexa Fluor® 488 Conjugate) (#4408 S) were from Cell Signaling Technology (Danvers, MA). RPMI1640, penicillin and streptomycin, 0.25% trypsin, fetal bovine serum (FBS), and heat-inactivated FBS were from Gibco Laboratories (Gaithersburg, MD). Halt™ Protease and Phosphatase Inhibitor Cocktail, Keratinocyte-SFM and BRFF-HPC1™ media were from Thermo Fisher Scientific (Waltham, MA). DC™ Protein Assay kit was from Bio-Rad (Hercules, CA). The Poly- L-Lysine, Lactate Assay Kit II, 5-(N,N-Dimethyl)amiloride hydrochloride (DMA) and GW4869 were from Sigma-Aldrich (St. Louis, MO).
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