Catecholamine Exposure in iPSC-Derived Cardiomyocytes

cEND and MyEND were treated with a physiological and supraphysiological CAT mix—corresponding concentration c1–c3—as determined from TTS patient serum, comp. Table 1, Epinephrine (Sigma-Aldrich, E4250), norEpinephrine (Sigma-Aldrich, A0937), and dopamine (Sigma-Aldrich, H8502), the respective cell culture media, for 24 h. Epinephrine and norEpinephrine were dissolved in 0.5 M HCl, and culture medium was used as a solvent for dopamine.
For CAT treatment of iPSC-derived cardiomyocytes, human iPSC-derived cardiomyocytes were treated with a physiological mix of these CATs, Epinephrine (Sigma-Aldrich, E4250), norEpinephrine (Sigma-Aldrich, A0937), and dopamine (Sigma-Aldrich, H8502),—corresponding concentration c3/ TTS acute levels as determined from TTS patient serum, comp. Table 1 in RPMI medium supplemented with 1× B-27 (Gibco, 17504001) for 24 h. Additionally to the CAT mix designated c3, cells were treated with a supraphysiological concentration of 5 mM isoprenaline (Sigma-Aldrich, I6504) [42 (link)].