Human pleural MM cell lines, H2373, H2595, and HP-1 were kindly contributed by Dr. Harvey Pass (New York University, New York, NY)48 (link) and Hmeso cells were isolated by Reale et al.49 (link). Human primary pleural mesothelial cells HPM3 and human immortalized peritoneal mesothelial LP9/TERT-1 (LP9) cells were purchased from Brigham and Women’s Hospital, Harvard University, Boston, MA.
All cell lines were cultured as previously reported50 (link). Cell lines were validated by STR DNA fingerprinting using the Promega CELL ID System (Promega, Madison, WI)50 (link).
Cisplatin was purchased from Alfa Aesar (Ward Hill, MA) and concentrations for the present study were selected based on previously published literature for MM cells51 (link). GW4869, Cl-amidine (chloramidine), and bisindolylmaleimide-I were purchased from Cayman Chemical (Ann Arbor, MI) and used at concentrations based on published reports indicating successful inhibition of exosome release from cells34 (link),35 (link). DMSO in equal volume added to control wells as vehicle control.
All cell lines were cultured as previously reported50 (link). Cell lines were validated by STR DNA fingerprinting using the Promega CELL ID System (Promega, Madison, WI)50 (link).
Cisplatin was purchased from Alfa Aesar (Ward Hill, MA) and concentrations for the present study were selected based on previously published literature for MM cells51 (link). GW4869, Cl-amidine (chloramidine), and bisindolylmaleimide-I were purchased from Cayman Chemical (Ann Arbor, MI) and used at concentrations based on published reports indicating successful inhibition of exosome release from cells34 (link),35 (link). DMSO in equal volume added to control wells as vehicle control.
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