Lipid and content mixing assays, using v- and t-SNARE liposomes, were carried out as previously described72 (link) except that the macromolecular crowding agent49 (link), Ficoll 70 (100 mg/ml, GE Healthcare), was included in the reconstitution buffer (25 mM HEPES, 100 mM KCl, pH 7.4). Data were obtained from three independent trials.
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