ChIP-seq Analysis of Histone H3K27 Acetylation
Corresponding Organization : Soochow University
Other organizations : First Affiliated Hospital of Bengbu Medical College
Variable analysis
- Formaldehyde concentration (1%)
- Formaldehyde incubation time (10 minutes)
- Glycine concentration (1.25 M)
- Glycine incubation time (5 minutes)
- H3K27ac enrichment (measured by ChIP-seq)
- Cell count (3-5 × 10^7 cells)
- Antibody used (anti-histone H3 (acetyl K27) antibody)
- Antibody incubation time (overnight at 4°C)
- DNA purification method (QIAquick PCR purification kit)
- Sequencing platforms (NovaSeq 6000, BGISEQ 2000)
- Alignment software (Bowtie2)
- Peak calling software (MACS2)
- Visualization software (Integrative Genomics Viewer)
- Superenhancer identification method (ROSE)
- Positive control: Not specified.
- Negative control: Not specified.
Annotations
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