Transgenic Mouse Strains for Immunological Research

Mice were maintained on a 12 light/ dark cycle in a barrier facility, had ad libitum access to food and water, were exclusively used for the purposes of this study, and had not undergone any additional procedures. Mating cages housed one male and two females of the same genotype, and males were separated from dams at the time of delivery. Injections were performed on entire litters of 0–3-day-old mice of the following strains: C57BL/6J, Rag1^−/− (B6.129S7-Rag1^tm1Mom/J; strain 002216, Jackson laboratories), Cxcl10^−/− (B6.129S4-Cxcl10^tm1Adl/J; strain 006087, Jackson laboratories), CD4-deficient (B6.129S2-Cd4^tm1Mak/J; strain 002663, Jackson laboratories), CD8-deficient (B6.129S2-Cd8^atm1Mak/J; strain 002665, Jackson laboratories), CD4/CD8-deficient (intercrossed strains 002663 and 002665), NOD/SCID (NOD.Cg-Prkdc^scid/J; strain 001,303, Jackson laboratories), Ccl5^−/− (B6.129P2-Ccl5^tm1Hso/J; strain 005090; Jackson laboratories) and Cx3cr1^−/−; Ccr2^−/− [47 (link)] mice. Injected pups were allowed to recover and were subsequently immediately returned to their maternal cage until weaning. Mice of both sexes were randomly assigned to all experimental groups without bias, and the investigators were blinded until final data analysis during all of the experiments.