Fabrication of Nafion-coated Carbon Fiber Microelectrodes

CFM’s were constructed through the aspiration of a single T-650 carbon fiber (7 μm, Goodfellow, Coraopolis, PA) through a cylindrical glass capillary (internal diameter: 0.4 mm, external diameter: 0.6 mm, Product # 624500, A-M Systems, Carlsborg, WA). This capillary was then placed in a vertical pipette puller (Narishige Group, Setagaya-Ku, Tokyo, Japan) to make a carbon – glass seal by gravity. The protruding carbon fiber was then cut to 150 μm in length. Subsequently, a solution of Nafion (LQ-1105-US-25, 5% by weight Nafion, Ion Power, DE) was electrodeposited, as previously described, onto the exposed carbon fiber (Hashemi et al. 2009 (link)). The CFM was then dried for 10 minutes at 70°C.

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Abdalla A., West A., Jin Y., Saylor R., Qiang B., Peña E., Linden D.J., Nijhout H.F., Reed M.C., Best J, & Hashemi P. (2019). Fast Serotonin Voltammetry as a Versatile Tool for Mapping Dynamic Tissue Architecture: I. Responses at Carbon Fibers Describe Local Tissue Physiology. Journal of neurochemistry, 153(1), 33-50.

Publication 2019

T-650 carbon fiber vertical pipette puller

Capillary Carbon Carbon fiber Gravity Nafion Seal

Corresponding Organization : University of South Carolina

Other organizations : Johns Hopkins Medicine, Johns Hopkins University, Southern Illinois University Edwardsville, Duke University, The Ohio State University

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Variable analysis

independent variables

Aspiration of a single T-650 carbon fiber (7 μm, Goodfellow, Coraopolis, PA) through a cylindrical glass capillary

dependent variables

Electrodeposition of a solution of Nafion (LQ-1105-US-25, 5% by weight Nafion, Ion Power, DE) onto the exposed carbon fiber

control variables

Cylindrical glass capillary (internal diameter: 0.4 mm, external diameter: 0.6 mm, Product # 624500, A-M Systems, Carlsborg, WA)
Vertical pipette puller (Narishige Group, Setagaya-Ku, Tokyo, Japan) to make a carbon – glass seal by gravity
Cutting the protruding carbon fiber to 150 μm in length
Drying the CFM for 10 minutes at 70°C

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