Antibodies for TRAIP, γH2AX, and DNA damage

The anti-TRAIP polyclonal antibodies were raised against GST tagged TRAIP-N terminal fusion proteins and were affinity-purified using column coated with N-terminal MBP tagged TRAIP as previously described (29 (link)). Anti-γH2AX antibodies were previously described (30 (link)). The anti-S9.6 antibodies were from Kerafast (Boston, USA); anti-Nucleolin (C23) antibodies were from Santa Cruz (Dallas, TX, USA); anti-Ki67 antibodies were from Chemicon (Darmstadt, Germany). (Z)-4-Hydroxytamoxifen (4-OHT), anti-Actin, anti-Flag (Rabbit) and anti-Flag (Mouse; Clone M2) antibodies were from Sigma (Darmstadt, Germany); anti-pRPA32 antibodies were from Bethyl Laboratories. Shield-1 was purchased from Takara (Kanagawa, Japan). Hydroxyurea, Aphidicolin, Mitomycin C, Cisplatin, Actinomycin D, α-Amanitin, and DRB were from Sigma (Darmstadt, Germany). CX5461, ATM inhibitor (ATMi; KU55933) and ATR inhibitor (ATRi; VE821) were from SelleckChem (Houston, TX, USA). DAPI (4′,6-diamidino-2-phenylindole) and DNase I (EN0523) was from Thermo Fisher Scientific. RNase A was from Qiagen.

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Chen Y., Li J., Cao F., Lam J., Cheng C.C., Yu C.H, & Huen M.S. (2018). Nucleolar residence of the seckel syndrome protein TRAIP is coupled to ribosomal DNA transcription. Nucleic Acids Research, 46(19), 10119-10131.

Publication 2018

anti-Ki67 antibodies (Z)-4-Hydroxytamoxifen (4-OHT), anti-Actin anti-Flag (Rabbit) Shield-1 Hydroxyurea Aphidicolin Mitomycin C Cisplatin Actinomycin D α-Amanitin CX5461 DAPI (4′,6-diamidino-2-phenylindole) RNase A

4 hydroxytamoxifen 4 oht Actin Actinomycin d Amanitin Anti antibodies Antibodies Aphidicolin Cisplatin Clone Cx5461 Dapi Dnase i Hydroxyurea Ku55933 Mitomycin c Mouse Nucleolin Proteins n Rabbit Rnase

Corresponding Organization : Chinese Academy of Sciences

Top 5 similar protocols

Variable analysis

independent variables

Anti-TRAIP polyclonal antibodies raised against GST tagged TRAIP-N terminal fusion proteins
Anti-γH2AX antibodies
Anti-S9.6 antibodies
Anti-Nucleolin (C23) antibodies
Anti-Ki67 antibodies
(Z)-4-Hydroxytamoxifen (4-OHT)
Anti-Actin antibodies
Anti-Flag (Rabbit) antibodies
Anti-Flag (Mouse; Clone M2) antibodies
Anti-pRPA32 antibodies
Shield-1
Hydroxyurea
Aphidicolin
Mitomycin C
Cisplatin
Actinomycin D
α-Amanitin
CX5461
ATM inhibitor (ATMi; KU55933)
ATR inhibitor (ATRi; VE821)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

Anti-TRAIP polyclonal antibodies affinity-purified using column coated with N-terminal MBP tagged TRAIP

negative controls

None specified

Annotations

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