Acetone and methanol extracts were dried under nitrogen and resuspended in ethanol. The absorbance (Ax) was measured at 445, 663 and 750 nm by a UV–visible spectrophotometer (Genesys 50, Thermo Scientific). Fucoxanthin concentrations, expressed as mg g−1 dry weight (DW), was estimated using the following equation [79 (link)]:
where:
W = sample weight (g of dry weight)
V = ethanol volume (L)
A455, A663, A750 = absorbance at x nm
where:
W = sample weight (g of dry weight)
V = ethanol volume (L)
A455, A663, A750 = absorbance at x nm
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