Protocol detail

Plasmid Constructs for Autophagy and Cytoskeleton Research

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pMXs-puro GFP-p62 (Addgene, 38277) (Itakura and Mizushima, 2011 (link)), pMXs-puro GFP-p62ΔC (addgene, 38282) (Itakura and Mizushima, 2011 (link)), ECFP-SSH1ΔC (Kurita et al., 2008 (link)), pEGFP-N1 human cofilin WT (addgene, 50859) (Garvalov et al., 2007 (link)), pEGFP-N1 human cofilin S3A (addgene, 50860) (Garvalov et al., 2007 (link)), pEGFP-N1 human cofilin S3E (addgene, 50861) (Garvalov et al., 2007 (link)), pOPTN-EGFP (addgene, 27052) (Park et al., 2006 (link)), pOPTN E478G-EGFP (addgene, 68848) (Turturro et al., 2014 (link)) were obtained from corresponding sources. Plasmids p3xFlag-SSH1 and p3xFlag-SSH1ΔN, and GFP-p62 403E were generated in the Kang lab as previously documented (Fang et al., 2021 (link)).

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Cazzaro S., Fang C., Khan H., Witas R., Kee T.R., Woo J.A, & Kang D.E. (2022). Slingshot homolog-1 mediates the secretion of small extracellular vesicles containing misfolded proteins by regulating autophagy cargo receptors and actin dynamics. Frontiers in Aging Neuroscience, 14, 933979.

Publication 2022

pMXs-puro GFP-p62 ECFP-SSH1ΔC pEGFP-N1 human cofilin WT pOPTN-EGFP

Cofilin Human Plasmids Ssh1

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Variable analysis

independent variables

PMXs-puro GFP-p62
PMXs-puro GFP-p62ΔC
ECFP-SSH1ΔC
PEGFP-N1 human cofilin WT
PEGFP-N1 human cofilin S3A
PEGFP-N1 human cofilin S3E
POPTN-EGFP
POPTN E478G-EGFP
P3xFlag-SSH1
P3xFlag-SSH1ΔN
GFP-p62 403E

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

Not specified

negative controls

Not specified

Annotations

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