PET Imaging of [18F]Fallypride Binding

PET imaging was collected at Vanderbilt University Medical Center. [¹⁸F]Fallypride was produced by the PET radiochemistry laboratory following the synthesis and quality control guidelines described in US Food and Drug Administration IND 47,245. A 5.0 mCi slow bolus injection of [18F]Fallypride was followed by three, 3D emission scans in a GE Discovery STE scanner (3.25mm axial slices with in-plane pixel dimensions of 2.3×2.3mm). The same scanner was used for both studies. Prior to each emission scan, CT scans were collected for attenuation correction. Scanning lasted for approximately 3.5 hours, with two 15-minute breaks for participant comfort. Decay, attenuation, motion, and partial volume correction was performed on the PET scans and voxelwise BP_ND maps, which represent the ratio of specifically-bound [¹⁸F]Fallypride to its free concentration, were calculated using the PMOD Biomedical Imaging Quantification software (see (Dang et al., 2016 (link); Smith et al., 2017 ) for greater detail).