Antibody Characterization for HBV Analysis

A rabbit anti-core antibody [36 (link), 67 (link)] (1:2000) was used for Western blot analysis, Phos-tag SDS-PAGE, native agarose gel electrophoresis (NAGE) (1:5000), and immunofluorescence analysis (IFA) (1:200). A rabbit anti-HBx antibody (Abcam, ab39716) (1:2000) was used for Western blot analysis of HBx protein. A goat anti-HBs antibody (B0560, Dako) (1:5000) was used for detecting HBs by NAGE. A mouse α-tubulin antibody (DM1A) (Genetex, GTX11302) (1:2000) was used for Western blot analysis. A mouse HA-tag antibody (Genetex, GTX115044) (1:1000) was used for immunoprecipitation assay (2 µg) and Western blot analysis. A mouse anti-Flag M2 antibody (Sigma, F1804) (1:2000) was used for immunoprecipitation assay (2 µg) and Western blot analysis.