Conditional Deletion of IGF-1 Signaling

C57BL/6J, Igf1r^fl/fl, and Igf1^fl/fl mice were obtained from Jackson Laboratories. CCSP-_rtTA/tetO-Cre were kindly provided by Dr. Jeffrey Whitsett at Cincinnati Children’s Hospital^{37 (link)}. To generate IGF-1R deletion in Club cells, we crossed CCSP-_rtTA/tetO-Cre mice to Igf1r^{fl/fl36 (link)}. To achieve deletion, mice were given doxycycline (1mg/mL) in drinking water containing 0.4% sucrose for at least 7 days prior to beginning of allergen administration, unless otherwise noted. We also crossed Igf1^fl/fl mice^{33 (link),34 (link)} to LysM-Cre mice to conditionally delete Igf1 in the myeloid lineage. We have previously reported on the generation of CCSP-Cre/YFP mice^{4 (link)}. For all in vivo experiments, except generation of apoptotic thymocytes, mice between the ages of 8 and 12 weeks were used. No blinding was performed for in vivo experiments. Mice were allocated to experimental groups based on genotype and age-matching. Female and male mice were used for all experiments, except for in vivo engulfment assays in which only male mice were used. Sample size was selected based on mice availability and power statistics. All animal procedures were performed according to the protocols provided by the Institutional Animal Care and Use Committee (IACUC) of the University of Virginia.