For experiments male B6.SJL-Ptprcapepcb/BoyJ mice (CD45.1+/+) 8–12 weeks of age were purchased from The Jackson Laboratory (Bar Harbor, ME). CD45.2+ B cells from male or female 09–1B12-UCA KI mice (H09–1B12-UCA/WT, κ09−1B12-UCA/WT) were enriched using the Pan B Cell Isolation Kit II (Miltenyi Biotec), counted, diluted to desired cell numbers in PBS and adoptively transferred into CD45.1+ recipient mice as reported previously43 (link). Ages of the recipient animals ranged from 8–10 weeks.
Ray R., Mohamed F.A., Maurer D.P., Huang J., Alpay B.A., Ronsard L., Xie Z., Han J., Fernandez-Quintero M., Phan Q.A., Ursin R.L., Vu M., Kirsch K.H., Prum T., Rosado V.C., Bracamonte-Moreno T., Okonkwo V., Bals J., McCarthy C., Nair U., Kanekiyo M., Ward A., Schmidt A.G., Batista F.D, & Lingwood D. (2024). Eliciting a single amino acid change by vaccination generates antibody protection against group 1 and group 2 influenza A viruses. Immunity, 57(5), 1141-1159.e11.
Other organizations :
Massachusetts General Hospital, Ragon Institute of MGH, MIT and Harvard, Scripps Research Institute, Harvard University, Universität Innsbruck, National Institute of Allergy and Infectious Diseases, National Institutes of Health
Genotype of donor B cells (H09-1B12-UCA/WT, κ09-1B12-UCA/WT)
dependent variables
Adoptive transfer of CD45.2+ B cells into CD45.1+ recipient mice
control variables
Age of recipient mice (8-10 weeks)
Sex of recipient mice (male)
Strain of recipient mice (B6.SJL-Ptprca pepc/BoyJ, CD45.1+/+)
controls
Positive control: Not specified
Negative control: Not specified
Annotations
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