HT22 cells were cultured in DMEM media supplemented with 10% FBS and 1% penicillin and streptomycin [14 (link)]. Western blots were carried out as previously reported [31 (link)] using the following primary and secondary antibodies: anti-acetylated-lysine antibody (1:1000; Cell Signaling, #9441), anti-acetyl α-tubulin-Lys40 antibody (1:10,000; 6-11B-1, Santa Cruz), anti-α-tubulin antibody (1:5000; Abcam, ab18251), anti-Tyr-Tub antibody (1:5000; Sigma T9028), anti-HDAC6 antibody (1:1000; Cell Signaling, #7558), anti-ATAT1 antibody (1:1000; Novus Biologicals, NBP1-57650), donkey anti-rabbit IgG-HRP (1:20,000; Santa Cruz) and donkey anti-mouse IgG-HRP (1:20,000; Santa Cruz). Immunostaining was performed using FITC-conjugated secondary antibodies (Jackson Immuno Research Labs) as we described previously [54 (link)]. Protein identification was conducted by Science Core Facility at Harvard University (http://proteomics.fas.harvard.edu/). Briefly, after in gel trypsin digestion, a sample was submitted for single LC-MS/MS experiment that was performed on a LTQ Orbitrap Elite (Thermo Fischer) equipped with Waters® NanoAcquity HPLC pump (Milford, MA). Raw data were submitted for analysis in Proteome Discoverer 2.1.0.81 (Thermo Fischer) software. Assignment of MS/MS spectra was performed using the Sequest HT algorithm by searching the data against UniprotKB/Swissprot database.