Specific pathogen free (SPF) mice were produced at each of the 3 test sites by mating CB6F1 females with C3D2F1 males to produce a genetically heterogeneous population15 (link). Weanlings at the 3 sites were fed similar diets (but not identical; see supplementary material for details) until they were started on food containing rapamycin, in Purina 5LG6, at 600 days of age. A second independent study was begun one year later, with rapamycin initiated at 270 days rather than at 600 days of age. The principal endpoint was age at death (for mice found dead at daily inspections) or age at euthanasia (for mice deemed unlikely to survive for more than an additional 48 hrs).
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Aging Study of Rapamycin in Mice
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Corresponding Organization :
Other organizations : Jackson Laboratory, The University of Texas Health Science Center at San Antonio, National Institutes of Health, University of Michigan–Ann Arbor, New York University, University of Florida, Wake Forest University, Ann Arbor VA Medical Center
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Variable analysis
independent variables
- Age at which mice were started on food containing rapamycin (600 days vs. 270 days)
- Age at death (for mice found dead at daily inspections)
- Age at euthanasia (for mice deemed unlikely to survive for more than an additional 48 hrs)
- Genetic background of the mice (CB6F1 females mated with C3D2F1 males)
- Diet of the weanling mice (similar but not identical across the 3 test sites)
- No positive or negative controls were explicitly mentioned in the provided information.
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