A blue laser light (SDL-473-200T, DreamLasers, China) was directed into an optical fiber (50μm diameter), and diverging light from the fiber end was collimated and refocused to a 500μm diameter spot using convex lenses. Laser power density at the focused spot was adjusted to 250 mW/mm2 (ref. 2 (link)) with a rotatable neutral density filter. The laser spot was aligned onto the callosal band. Laser illumination lasted 2 ms and was controlled with a high-speed shutter (LS3T2, Uniblitz, USA). A small fraction of laser output was monitored with a photodiode (PDA100A, ThorLabs, UK). The illumination commenced 1 s after each condition started. The interstimulus interval for laser was > 2.5 s.
Protocol detail
Optical Stimulation of Callosal Pathway
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Callosal
Illumination
Lenses
Corresponding Organization : University College London
Other organizations : Wolfson Foundation
Top 5 similar protocols
Variable analysis
independent variables
- Laser power density at the focused spot
- Measured outcomes (not explicitly mentioned)
- Laser light (SDL-473-200T, DreamLasers, China)
- Optical fiber (50μm diameter)
- Convex lenses used to collimate and refocus the diverging light from the fiber end
- Rotatable neutral density filter to adjust the laser power density
- Laser spot aligned onto the callosal band
- Laser illumination duration (2 ms)
- Laser illumination controlled with a high-speed shutter (LS3T2, Uniblitz, USA)
- Laser output monitored with a photodiode (PDA100A, ThorLabs, UK)
- Interstimulus interval for laser (> 2.5 s)
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!