Antibody-based analysis of kidney injury

Monoclonal antibody for E–cadherin was purchased from BD Biosciences. Mac–1 antibody was purchased from AbD Serotec. F4/80 and BMP7 antibody was purchased from Abcam. p–Smad1/5 antibody was purchased from Cell Signaling Technology. Alk3 and E–cadherin antibodies were purchased from Santa Cruz Biotechnology. For western blot analyses, Alk3 antibody was purchased from EMD Millipore. Measurements of BUN were performed using the QuantiChrom™Urea Assay Kit (BioAssay System) or DIUR–500 QuantiChrom™ Urea Assay Kit (Gentaur). Measurements of urine albumin and urine creating were performed using the QuantiChrom™ Assay for albumin (DIAG-500) and creatinine (DICT-250) Kit (BioAssay System). Details for morphormetric analyses of histological findings are listed in the online method section.

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Sugimoto H., LeBleu V.S., Basukonda D., Keck P., Taduri G., Bechtel W., Okada H., Carlson W J.r., Bey P., Rusckowski M., Tampe B., Tampe D., Kanasaki K., Zeisberg M, & Kalluri R. (2012). Activin–like kinase–3 activity is important for kidney regeneration and reversal of fibrosis. Nature medicine, 18(3), 396-404.

Publication 2012

Albumin Antibodies Antibody Bioassay Bmp7 Bun measurements Cadherin Creatinine E cadherin Mac 1 Monoclonal antibody Urea Urine Western blot analyses

Corresponding Organization :

Other organizations : Beth Israel Deaconess Medical Center, Harvard University, Massachusetts General Hospital, University of Massachusetts Chan Medical School, University of Göttingen

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Protocol cited in 6 other protocols

Variable analysis

independent variables

Monoclonal antibody for E-cadherin
Mac-1 antibody
F4/80 antibody
BMP7 antibody
P-Smad1/5 antibody
Alk3 antibody
E-cadherin antibody

dependent variables

Measurements of BUN
Measurements of urine albumin
Measurements of urine creatinine
Morphometric analyses of histological findings

control variables

Not explicitly mentioned

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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