Intranasal SARS-CoV-2 Infection in Mice

BALB/c mice (four-week-old, female) were purchased from Japan SLC (Shizuoka, Japan). The mice were divided into three groups with nine animals in each group. Mice were anesthetized and inoculated intranasally (20 µL) of rD/OK or rD/OK-AL (1.0 × 10⁵ PFU), respectively. Organs containing nasal turbinates, the tracheas, and the lungs were collected after three and six days following inoculation and homogenized in phosphate-buffered saline (PBS) for virus titration via the plaque assay.

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Ishida H., Murakami S., Kamiki H., Matsugo H., Katayama M., Sekine W., Ohira K., Takenaka-Uema A, & Horimoto T. (2023). Generation of a recombinant temperature-sensitive influenza D virus. Scientific Reports, 13, 3806.

Publication 2023

BALB/c mice

Animals Balb c mice Female Inoculation Lungs Mice Nasal turbinates Phosphate Saline Titration Tracheas Virus plaque assay

Corresponding Organization :

Other organizations : The University of Tokyo

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Variable analysis

independent variables

Intranasal inoculation of rD/OK or rD/OK-AL (1.0 × 10^5 PFU)

dependent variables

Virus titers in nasal turbinates, tracheas, and lungs

control variables

BALB/c mice (four-week-old, female)
Mice divided into three groups with nine animals in each group
Organ samples collected at three and six days following inoculation

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