The site of animal electroacupuncture intervention was consistent with those used in previous experiments (Wang et al., 2015 (link)). Zhong-wan-acupoint (RN12) was located on the intersection of the upper 1/3 and lower 2/3 of the line connecting the xiphoid process and the upper border of the pubic symphysis, wei-shu-acupoint (BL21) was 2 mm adjacent to the spinous process of the 12th thoracic vertebra. Using disposable sterile acupuncture needles (0.25*13 mm, Yunlong Medical Co., Ltd., China) and electrical stimulator (G6805, Qingdao Xinsheng, China). A 20 min EA procedure was performed at a frequency of 2/100 Hz by using current with an intensity of 0.1–1 mA.
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