AST was performed by the broth microdilution method using the VITEK 2 Compact System (BioMerieux, Lyon, France). The antimicrobial agents tested included piperacillin/tazobactam (TZP), cefoxitin (FOX), cefuroxime (CXM), ceftazidime (CAZ), cefotaxime (CTX), cefepime (FEP), imipenem (IPM), meropenem (MPN), amikacin (AMK), gentamicin (GEN), ciprofloxacin (CIP), levofloxacin (LEV), tetracycline (TE), trimethoprim/sulfamethoxazole (STX), chloramphenicol (C), and aztreonam (ATM), The minimum inhibitory concentrations (MICs) were interpreted according to the recommendations of the Clinical and Laboratory Standards Institute (CLSI) for Aeromonas spp. (Institute, 2015 ) E.coli ATCC25922 was used as the quality-control strain. Multidrug resistance (MDR) was defined as non-susceptibility to at least one agent in three or more antimicrobial categories (Magiorakos et al., 2012 (link)).
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