Quantitative Gene Expression Analysis
Corresponding Organization :
Other organizations : Oxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, Stanford University, University of Alberta, Oxford BioMedica (United Kingdom)
Variable analysis
- RNA extraction method (TRIzol reagent for EndoC-βH1, PicoPure RNA isolation kit for primary human pseudoislets)
- CDNA synthesis method (GoScript Reverse Transcriptase System for EndoC-βH1, Maxima first strand cDNA synthesis kit for primary human pseudoislets)
- Gene expression measured by quantitative qPCR (qPCR)
- CALCOCO2 expression extracted from previously published RNA-seq data
- Housekeeping genes (PPIA, GAPDH, and TBP) used for normalization of target gene expression
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
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