Fresh ovules of Arabidopsis were dissected from siliques using forceps and mounted in Hoyer's solution [chloral hydrate: glycerol: water, 8:1:2 (w/v/v)] for 30 min or 6–8 h depending on the embryo developmental stage (Chen et al., 2015 (link)). Then, the cleared ovules were observed and photographed with differential interference contrast microscopy (Olympus TH4-200 equipped with a CCD of a SPOT digital microscope camera).
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