All experiments were performed on a lab-built Raman microscope as previously reported.42 (link) The key components are a 17 mW (cw) 632.8 nm HeNe laser, a spectrograph (Shamrock 303i, Andor) with a 600 gr/mm grating, and a EMCCD (Newton 970, Andor). A 40x water immersion objective (Olympus, NA=0.8) was used for excitation and collection of the Raman signal. The laser power measured at the sample was 1 mW.
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Raman Microscopy for Molecular Analysis
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Corresponding Organization : Notre Dame of Dadiangas University
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Variable analysis
independent variables
- Laser power
- Raman signal
- Laser wavelength (632.8 nm)
- Laser mode (cw)
- Laser power (1 mW at the sample)
- Spectrograph (Shamrock 303i, Andor)
- Grating (600 gr/mm)
- Detector (EMCCD Newton 970, Andor)
- Objective (40x water immersion, Olympus, NA=0.8)
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