Dual Secreted Luciferase Assay for ATF2 and c-JUN

To assess ATF2 and c-JUN transcriptional activity, we implemented a dual secreted luciferase assay as previously described (20 (link)). At 48 hours after transfection, the cells were treated with specified drugs, and at the indicated times, media samples containing secreted lucifase were harvested and measured for luciferase activity using the Pierce Gaussia Luciferase Glow Assay Kit per manufacturer’s instructions (ThermoFisher).

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Emmons M.F., Faião-Flores F., Sharma R., Thapa R., Messina J.L., Becker J.C., Schadendorf D., Seto E., Sondak V.K., Koomen J.M., Chen Y.A., Lau E.K., Wan L., Licht J.D, & Smalley K.S. (2019). HDAC8 regulates a stress response pathway in melanoma to mediate escape from BRAF inhibitor therapy. Cancer research, 79(11), 2947-2961.

Publication 2019

Pierce Gaussia Luciferase Glow Assay Kit

Assay Atf2 C jun Cells Drugs Luciferase Transcriptional Transfection

Corresponding Organization : Moffitt Cancer Center

Other organizations : George Washington University, University of Florida Health

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Variable analysis

independent variables

Specified drugs

dependent variables

Luciferase activity

control variables

Time points of media sample collection

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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