Fluorescence Immunostaining of Tyrosine Hydroxylase

Immunohistochemistry was performed as described previously (Bateup et al., 2013 (link)). The following antibodies were used: tyrosine hydroxylase (TH, ImmunoStar #22941, RRID:AB_572268), Alexa Fluor 488 goat anti-mouse secondary (Thermo Fisher Scientific #A-11001, RRID:AB_2534069), Alexa Fluor 633 goat anti-mouse secondary (Thermo Fisher Scientific #A-21050, RRID:AB_2535718), streptavidin Alexa Fluor 488 conjugate (Thermo Fisher Scientific #S32354, RRID:AB_2315383), and streptavidin Alexa Fluor 633 conjugate (Thermo Fisher Scientific #S21375, RRID:AB_2313500).

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Kramer D.J., Risso D., Kosillo P., Ngai J, & Bateup H.S. (2018). Combinatorial Expression of Grp and Neurod6 Defines Dopamine Neuron Populations with Distinct Projection Patterns and Disease Vulnerability. eNeuro, 5(3), ENEURO.0152-18.2018.

Publication 2018

A-11001 A-21050 S32354 S21375

Alexa fluor 488 Antibodies Goat Immunohistochemistry Mouse Streptavidin Tyrosine hydroxylase

Corresponding Organization :

Other organizations : University of California, Berkeley, Cornell University

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Variable analysis

independent variables

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dependent variables

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control variables

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controls

Positive control: The study by Bateup et al. (2013) is referenced, which likely included appropriate positive controls.
Negative control: No negative controls are explicitly mentioned.

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