Antibody Labeling in Alzheimer's Research

The following antibodies were used for labeling: affinity-purified rabbit polyclonal anti-APH-1a (B80.3) and affinity-purified monoclonal anti-NCT-CT 9C3 (Annaert et al., 2001 (link); Esselens et al., 2004 (link)); affinity-purified rabbit polyclonal against N-terminal amino acids 103–124 of NCT (anti-NCT-ECD, ab24741) and affinity-purified rabbit polyclonal against 13 amino acids at the C-terminus of PEN-2 (anti-PEN-2, ab62514) (both from Abcam); rabbit polyclonal against C-terminal amino acids 450–467 of PS-1 (anti-PS1-CT, S182; Sigma). The following antibodies were used for detection by western blotting: rabbit anti-PEN-2 (B126.1; Annaert et al., 2001 (link); Esselens et al., 2004 (link)); anti-human-PS1-loop (MAB5232, Chemicon); mouse anti-NCT (BD Transduction Laboratories); rat anti-PS1-NTF (MAB1563, Millipore); and anti-FLAG M2 (Sigma).