Intracellular Injection Method for Amacrine Cells
Corresponding Organization :
Other organizations : University of California, Los Angeles, VA Greater Los Angeles Healthcare System
Variable analysis
- Intracellular injections of Lucifer Yellow and Neurobiotin into amacrine cell bodies located in the proximal INL at the border of the IPL
- Bistratified morphology of the AII amacrine cell
- Borosilicate glass electrodes (#60200; A-M Systems; Sequim, WA, USA)
- 0.5% Lucifer Yellow (Sigma–Aldrich)
- 4% N-(2-aminoethyl)-biotinamide hydrochloride (Neurobiotin; Vector Laboratories, Burlingame, CA, USA)
- 0.1 M Tris buffer, pH 7.4
- 4% PFA fixation for 10 min
- 0.1 M PB washing for 30 min
- Streptavidin–FITC (1:500; Jackson ImmunoResearch, West Grove, PA, USA) incubation overnight at 4°C in 0.1M PB containing 0.3% Triton X-100 (Sigma–Aldrich)
- 3 times 30 min PB washing
- None specified
- None specified
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!