Intracellular injections were performed as described previously (Pérez de Sevilla Müller et al., 2007 (link), 2010a (link),b (link); Vuong et al., 2015 (link)). Borosilicate glass electrodes (#60200; A-M Systems; Sequim, WA, USA) were pulled and filled at their tips with 0.5% Lucifer Yellow (Sigma–Aldrich) 4% N-(2-aminoethyl)-biotinamide hydrochloride (Neurobiotin; Vector Laboratories, Burlingame, CA, USA), and back-filled with 0.1 M Tris buffer, pH 7.4. In retinal whole mounts, amacrine cell bodies located in the proximal INL at the border of the IPL were targeted for injection. Lucifer Yellow was iontophoresed (−1 nA) into a single cell body and when the bistratified morphology of the AII amacrine cell was recognized, the polarity of the current was reversed (+1 nA) and Neurobiotin was injected for 3 min. The retinas were then fixed in 4% PFA for 10 min and washed for 30 min in 0.1 M PB. Neurobiotin was visualized by incubating the retinas with the injected cells overnight at 4°C with streptavidin–FITC (1:500; Jackson ImmunoResearch, West Grove, PA, USA) in 0.1M PB containing 0.3% Triton X-100 (Sigma–Aldrich). Retinas were washed in PB three times for a total of 30 min. The retinas were subsequently processed for immunohistochemical staining.
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