For live-cell imaging, BSR cells were seeded onto 35-mm μdishes (Ibidi) 24 h before infection. Cells were infected with CVS-N2C-PmCherry rabies virus in DMEM fluorobrite medium (Invitrogen) supplemented with 5% FCS. Live-cell time-lapse experiments were recovered with a Zeiss AxioObserver epifluorescence microscope (63× oil-immersion objective). Cells were maintained at 37 °C and 5% CO2 during imaging.
For live imaging of stress granules, U373-MG cells were transfected using Lipofectamine 2000 (Invitrogen) with a plasmid encoding a G3BP-GFP fusion protein prior to cell infection as previously described20 (link).
For live imaging of stress granules, U373-MG cells were transfected using Lipofectamine 2000 (Invitrogen) with a plasmid encoding a G3BP-GFP fusion protein prior to cell infection as previously described20 (link).
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