As previously described (32 (link)), data of the scanned image files hybridized with probes from RNA extracted from the gastrocnemius muscle isolated at the specified times from the 2-AA-treated and untreated control mice (n=3) were converted to cell intensity files (CEL files) with the Microarray suite 5.0 (MAS; Affymetrix). The data were scaled to a target intensity of 500, and all possible pairwise array comparisons of the replicates to normal control mice were performed using a MAS 5.0 change call algorithm. Probe sets that had a signal value difference >100 and for which one of the two samples being compared was not termed 'absent', were scored as differentially modulated when i) the number of change calls in the same direction were at least 3, 4 and 6, when the number of comparisons were 4, 6 and 9, respectively; and ii) the other comparisons were unaltered. Based on the ratios of 100 genes determined to be invariant in most conditions tested (Affymetrix), an additional constraint of a minimum ratio of 1.65 was applied to control the known false positives at 5%. The microarray data are available at http://www.ncbi.nlm.nih.gov/geo/info/linking.html and the accession number is GSE43779. Gene Ontology (GO) analysis was performed using GeneSpring GX software (version 11) by Agilent Technologies (Santa Clara, CA, USA).