Lumbar puncture and CSF handling followed a structured protocol as previously described (39 (link)). CSF Aβ42 and Aβ40 were analyzed using Euroimmun ELISAs (EUROIMMUN AG, Lübeck, Germany) and p-tau181 was analyzed using Innotest ELISA (Fujirebio Gent, Belgium). Due to the biomodal distribution the CSF Aβ42/Aβ40 ratio mixture modeling was used to identify an unbiased cutoff for abnormal Aβ accumulation (<0.878), as previously described (40 (link),41 (link)).
MR imaging was performed at the same Siemens Trio 3 T system in all individuals and included transversal T2 FLAIR and a high-resolution isotropic MPRAGE. Automated segmentation of WML using the LST toolbox implemented in SPM8, generated a total lesion volume (mL), here named “WML volume,” for each individual (42 (link)).
MR imaging was performed at the same Siemens Trio 3 T system in all individuals and included transversal T2 FLAIR and a high-resolution isotropic MPRAGE. Automated segmentation of WML using the LST toolbox implemented in SPM8, generated a total lesion volume (mL), here named “WML volume,” for each individual (42 (link)).
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