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Electroretinography in Drosophila Larvae

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ERG was recorded in 1-day old flies using the same methods as previously described (Fabian-Fine et al., 2003 (link); Williamson et al., 2010 (link)). Briefly, flies were glued on glass slides using Elmer’s non-toxic glue. Both the reference and recording electrodes were made of glass pipettes filled with 3 M KCl. The light stimulus was computer-controlled using white light-emitting diode system (MC1500; Schott), and was provided in 1-s pulses. The data was recorded using Clampex software (version 10.1; Axon Instruments) and measured and analyzed using Clampfit software (version 10.2; Axon Instruments).

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Yusuff T., Chang Y.C., Sang T.K., Jackson G.R, & Chatterjee S. (2023). Codon-optimized TDP-43 mediates neurodegeneration in a Drosophila model of ALS/FTLD. Frontiers in Genetics, 14, 881638.

Publication 2023

MC1500 Clampex software Clampfit software

Axon Flies Light Pulses

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