Quantitative RT-PCR Analysis of IGF2R and HCN1

qRT-PCR was used to detect IGF2R and HCN1 mRNA expression levels and the corresponding primers are presented in S18 Table. qRT-PCR was performed using the SYBR PrimeScript RT-PCR Kit (TaKaRa, Dalian, China) with SYBR Green dye as described previously [60 (link)]. Briefly, qRT-PCR reactions were carried out with an iCycler IQ5 Multicolor Real-Time PCR Detection System (Bio-Rad, USA). The qRT-PCR reaction volume was 25 μL, contained 1 μL of cDNA template, 12.5 μL of SYBR Premix ExTaq, 9.5 μL of sterile water, and 1 μL of each gene-specific primer. Thermal cycling parameters were 1 cycle at 95°C for 2 min, 40 cycles of 95°C for 15 s, and 60°C for 34 s. Dissociation curve analysis was done after each real-time reaction to ensure that there was only one product. The qRT-PCR analysis of each sample was done in triplicate.