Comprehensive Glioma Cell Line Characterization

In the cellular uptake experiments, F98, C6 rat glioma, and 9L rat gliosarcoma cell lines were used. Each cell was provided by or purchased from as follows: F98 glioma cells, Dr. Rolf Barth (Ohio State University, Columbus, OH, USA); C6 glioma cells, the Japan Collection of Research Bioresources (JCRB) Cell Bank, National Institute of Biomedical Innovation (Osaka, Japan); 9L rat gliosarcoma cells, the American Type Culture Collection (ATCC; Manassas, VA, USA). All the cells were cultured in the medium that was used in our laboratory [16 (link),32 (link),33 (link),34 (link),35 (link),36 (link)]. This medium contained Dulbecco’s modified Eagle’s medium (DMEM) and was supplemented with 10% fetal bovine serum, penicillin, streptomycin, and amphotericin B. All the materials for making this culture medium were purchased from Gibco Invitrogen (Grand Island, NY, USA). Of these cell lines, F98 glioma cells are particularly useful in evaluating therapeutic agents [16 (link),32 (link),33 (link),34 (link),35 (link),36 (link)] because they simulate the behavior of human malignant gliomas in intracerebral implantation, including their high-infiltrative growth pattern and low immunogenicity [37 (link),38 (link)]. The F98 glioma-bearing rat brain tumor model was used for in vivo evaluation.