Curcumin Induces Apoptosis and Cell Cycle Alterations

LECs treated with different concentrations of curcumin for 48 h were collected and washed with PBS; cell apoptosis and cell cycle were analyzed by flow cytometry (Millipore, Darmstadt, Hesse, Germany) with the MultiCaspase Kit and the Cell Cycle Kit (Millipore, Darmstadt, Hesse, Germany). All procedures were proceeded according to the manufacturer's protocols as we previously did [21 (link), 22 (link)]. The statistical analysis was performed with the software version 1.3 of the Muse™ Cell Analyzer (Millipore, Darmstadt, Hesse, Germany).

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Liu H., Mao Y., Xia B., Long C., Kuang X., Huang H., Ning J., Ma X., Zhang H., Wang R., Tang H., Du H., Yan J., Zhang Q., Zhang X, & Shen H. (2020). Curcumin Inhibits Proliferation and Epithelial-Mesenchymal Transition in Lens Epithelial Cells through Multiple Pathways. BioMed Research International, 2020, 6061894.

Publication 2020

flow cytometry MultiCaspase Kit Cell Cycle Kit Muse™ Cell Analyzer

Apoptosis Cell Cell cycle Curcumin Flow cytometry Muse

Corresponding Organization : Sun Yat-sen University

Other organizations : Hunan Cancer Hospital, Central South University, Lanzhou University

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Variable analysis

independent variables

Different concentrations of curcumin

dependent variables

Cell apoptosis
Cell cycle

control variables

Cell type (LECs)
Incubation time (48 h)
Procedures (Millipore protocols)

controls

Positive control: Not specified
Negative control: Not specified

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