Antibody Sourcing and Preparation

DMSO, MG132, and Chloroquine were obtained from Sigma-Aldrich (St. Louis, MO, USA). Anti-Flag (M2), anti-HA, and anti-Myc antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-Tubulin antibodies and Protein A beads were obtained from Sigma-Aldrich. Antibodies against SGK1 (AF3200) were purchased from R&D Systems (Minneapolis, MN, USA). Antibodies against Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) and horseradish peroxidase-conjugated secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Fluorescein-and rhodamine-conjugated secondary antibodies were purchased from Jackson Immuno Research Laboratories (West Grove, PA, USA). Polyclonal mouse sera against PFV Gag, Tas, or Bet were prepared as described previously (82 (link)). The antibodies to BFV-Gag and BTas were also produced as described previously (22 (link), 84 (link)).