Mouse-derived macrophage Raw264.7 was purchased from Shanghai Yuanzhixin Biotechnology Co (Shanghai, China). Mouse bone marrow-derived macrophages were primarily extracted from wild-type Balb/c mice, and the methods have been detailed previously [5 (link)]. Mouse aortic endothelial cells (MAECs) were purchased from Otwo Biotech Co (Shenzhen, China). CD68 antibodies (ab125212), inducible nitric oxide synthase (iNOS) antibodies (ab210823), CD31 antibodies (ab24590), α-SMA antibodies (ab21027), Collagen-I antibodies (ab260043), VE-Cadherin antibodies (ab33168), GADPH antibodies (ab9485), Alexa Fluor® 488-labeled donkey anti-mouse secondary antibody (ab150109), Alexa Fluor® 555-labeled donkey anti-rabbit secondary antibody (ab150062) were purchased from Abcam Co (Cambridge, MA, USA); Vimentin antibodies (10366-1-AP), F4/80 antibodies (28463-1-AP), CD206 antibodies (60143-1-Ig), Interferon-γ (IFN-γ) were purchased from Proteintech Co (Chicago, IL, USA); CD206 Cell Flow antibodies (17-2061-80), lipopolysaccharide (LPS; 00⁃4976⁃93) were purchased from Thermo Fisher Scientific Co (Waltham, MA, USA); iNOS Flow Cytology antibodies (130-116-421) was purchased from Miltenyi Biotec Co (Bergisch Gladbach, Germany); The primers were designed and synthesized by Nanjing DynaScience Biotechnology Co. TNF-α Antagonist III (R-7050; 303997-35-5) was purchased from Selleck. Co (Shanghai, China).
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