Visualizing Na+ Distributions in Plant Roots

To visualize the Na⁺ distributions in the root cells of Col-0, OE lines and two SALK_119330 individual plants, the intracellular Na⁺ specific fluorescent indicator CoroNa-Green AM (Invitrogen Corp, Carlsbad, CA, USA) was used. Five-day-old seedlings grown on 1/2 MS medium were transferred to fresh medium containing 0 mM (control) or 150 mM NaCl for 72 h. The seedlings were then washed 2–3 times with distilled water and stained with 10 μM CoroNa-Green AM in the presence of pluronic acid (0.02%, Invitrogen) for 3 h. The incubated sections were visualized under an LSM700 microscope (Zeiss, Jena, Germany) at excitation and emission wavelengths of 488 and 516 nm, respectively, as described by Oh et al. (2010) (link). The experiments were conducted with three independent biological replications.