Human pancreatic adenocarcinoma cell lines CaPan-1, CaPan-2, AsPC-1, BxPC-3, Hs766T, MiaPaCa-2, PANC-1, PANC-28, PANC-48, PaTu8902, FG, Colo357 and L3.7, and gastric cancer cell lines GT5 and N87 were purchased from the American Type Culture Collection (Manassas, VA) or obtained as described previously (21 (link)). The HCT-116 parental colorectal cells and the cells with DNA methyltransferase 1 genetic disruption (HCT-116-DT1-KO) were gifts from Dr. Bert Vogelstein (The Johns Hopkins University School of Medicine, Baltimore, MD). All cancer cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) or otherwise described.
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Adenocarcinoma
Cancer
Cell lines
Disruption
Dna methyltransferase
Eagle
Fetal bovine serum
Gastric cancer
Gifts
Hct 116 cells
Human
Lines 1
Medicine
Pancreatic
Parental
Corresponding Organization : The University of Texas MD Anderson Cancer Center
Other organizations : Shanghai East Hospital
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Variable analysis
independent variables
- None explicitly mentioned
- None explicitly mentioned
- Cell lines used: CaPan-1, CaPan-2, AsPC-1, BxPC-3, Hs766T, MiaPaCa-2, PANC-1, PANC-28, PANC-48, PaTu8902, FG, Colo357, L3.7, GT5, N87, HCT-116 parental, and HCT-116-DT1-KO
- Culture media: Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (FBS)
- None explicitly mentioned
- None explicitly mentioned
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